Partial Purification of ß-Glucanase from Fruiting Bodies of Straw Mushroom (Volvariella volvacea) by Aqueous Two Phase Extraction and Its Hydrolysis of Mushrooms Cell Walls

The objective of this study was to partially purify ß-glucanase from crude enzyme extract of straw mushroom (Volvariella volvacea). The crude enzyme extract was prepared from the fruiting bodies of straw mushroom. The ßglucanase was partially purified using an aqueous two-phase extraction with Triton X-114 and followed by ammonium sulfate precipitation in the range of 50-80% saturation. The ß-glucanase activity was increased 4.1-fold with recovery yield of 70%. The phenolic compound was also reduced to 7.2% of the original content, avoiding the post purification tanning of the enzyme. Furthermore, the ß-glucanase was able to hydrolyze V. volvacea cell-wall and other mushrooms cell-walls such as oyster mushroom (Pleurotus sajor-carjo) and ear mushroom (Auricularia auricular).